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<p><b>OBJECTIVE</b>To explore the characteristics of Helicobacter pylori (H. pylori) antigen in serum and to evaluate its clinical diagnostic value.</p><p><b>METHODS</b>Enzyme-linked immunosorbant assay (ELISA) was developed to detect the soluble H. pylori antigen (S-Hp) and circulatory specific H. pylori antigen immunocomplexes (Hp-IC) in serum.</p><p><b>RESULTS</b>The positive rate of S-Hp was 90.91% from 66 patients with H. pylori infection, which was much greater than 0% found in 28 controls (P < 0.001). Moreover, its concentration closely reflected the number of H. pylori in the gastric mucosa layer. We also found that Hp-IC existed bound with IgG and/or IgA in patients with positive S-Hp. However, there is no evidence to show the concentration of S-Hp reduced significantly in followed-up subjects after effective therapy.</p><p><b>CONCLUSIONS</b>These methods as newly and noninvasive complementary tools can be used for clinical diagnosis of H. pylori infection. In addition, S-Hp and Hp-IC may be of importance in H. pylori pathogenesis.</p>
Subject(s)
Adult , Female , Humans , Male , Antibody Specificity , Antigen-Antibody Complex , Blood , Antigens, Bacterial , Blood , Helicobacter Infections , Allergy and Immunology , Helicobacter pylori , Allergy and Immunology , Immunoglobulin A , Blood , Immunoglobulin G , BloodABSTRACT
Objective To study whether omeprazole induces gastric epithelial cells apoptosis and analyse the possible mechanism. Methods The cell cycle and apoptosis ratio of SGC 7901 cell line were determined by flow cytometry in the presence of omeprazole, meanwhile, TUNEL positive cells from gastric mucosa were counted after incubated with omeprazole for 72 hours. Expression of p53、 GADD 45 、 p15,16,18,19 and bcl 2 were stained by immunohistochemical assay and ATPase activity was tested. Results significant alteration with decreasing percentage of G 1 phase and increasing percentage of G 2 phase of SGC 7901 cell line could be detected after incubating 24 hours with omeprazole at double clinical doses . An apoptosis peak appeared in flow cytometry after incubating 72 hours with omeprazole. However, no genetic products such as p53, GADD 45 , p15,16,18,19 and bcl 2 were seen. Concentrations of ATPase were markedly increased from 0.326 0.387 before incubated with omeprazole to 0.054 0.103 after incubated with omeprazole. TUNEL positive reaction cell counts in fresh mucosa was also increased after incubated with omeprazole for 72 hours( P
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Objective To study the influence of bcl-2 on gastric tumorigenesis and its significance.Methods The expression was observed in 20 normal gastric mucosa, 38 gastric epithelial dysplasia(GED)and 52 gastric adenocarcinomas (GAC) by immunohistochemical method. Results in bcl-2 expression,significant difference was found between GED and normal gastric mucosa (P
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0.05),However,the amount of metronidazole efflux was significantly decreased after adding verapamil(P
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Objective To study the effects of different concentrations of antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu8988s. Methods Human pancreatic cancer cell line PaTu8988s in exponent growth stage are adopted. We observe the effect of different time point on the PaTu8988s cell at 12、24、48 and 72 hour. Results The inhibitory rate on PaTu8988s cell line is 42.25%、66.29%、69.55%、74.58% and 66.20%、91.43%、98.18%、98.33% for ASODN concentrations of 50 ?g/ml and 100 ?g/ml at 12、24、48 and 72 hour, respectively. Conclusion The inhibitory effect of ASODN began from 12 hour and becomes more obvious at 48～72 hour. The higher the concentration of ASODN, the earlier the peak of inhibited rate.